GOST 10444.11-89 PDF

Full title and description

GOST 10444.11-89 — Food products. Methods for determination of lactic acid bacteria (Продукты пищевые. Методы определения молочнокислых микроорганизмов). The standard specifies laboratory methods for detecting and enumerating lactic acid bacteria in foodstuffs, with particular provisions for dairy and canned products.

Abstract

This national/interstate standard establishes microbiological procedures for sampling, preparation, media, reagents and test methods used to detect and quantify mesophilic lactic acid bacteria in food products. Methods include inoculation in sterile milk with MPN (most probable number) assessment and culture on solid/semisolid nutrient media with subsequent colony counts and identification-related steps.

General information

• Status: Superseded / replaced by a later edition (GOST 10444.11-2013 / GOST 10444.11-2013 implemented subsequently).
• Publication date: Approved 28 November 1989 (designation suffix -89).
• Publisher: Gosstandart (USSR) / Izdatel'stvo standartov (Standards Publishing houses that issued the GOST texts).
• ICS / categories: 67.100.10 — Milk and milk products; falls under food microbiology classifications.
• Edition / version: Original edition (1989), later replaced by the 2013 revision (GOST 10444.11-2013).
• Number of pages: 14 pages (typical printed/pdf edition).

Scope

The standard applies to food products generally and gives special instructions for dairy and fermented-milk products, concentrates and bacterial preparations of lactic acid bacteria. It covers sample collection and preparation, preparation of dilutions, required apparatus and reagents, incubation conditions and interpretative rules for results when determining lactic acid bacterial counts by MPN and plate-count techniques. The standard is intended for use where quantitative control of lactic acid bacteria is required.

Key topics and requirements

• Scope and application to dairy products, fermented foods and canned goods.
• Rules for sampling and preparation of test samples (including special rules for concentrated and high-salt products).
• Preparation and composition of diluents and culture media; sterilization and quality requirements for reagents and media.
• Two primary enumeration techniques: inoculation into sterile milk with MPN estimation and plating on solid or semisolid nutrient media with colony counts.
• Specified incubation temperatures and times, colony reading and calculation procedures for results.
• Required laboratory apparatus, sterility controls and reporting conventions for microbiological counts.

Typical use and users

The standard is used by food microbiology laboratories, quality control departments in dairy and food manufacturing, regulatory and inspection bodies, and research laboratories involved in product validation or shelf-life studies. It provides established methods for routine monitoring of lactic acid bacteria levels in production, incoming materials and finished products.

Related standards

Often used alongside other GOSTs referenced in the norm, for example GOST 10444.1 (general microbiological methods and apparatus), GOST 9225 (sampling and preparation for dairy products), GOST 26669 (diluents and sample preparation), and other parts of the GOST 10444.x series that cover different microbial groups and methods. The 1989 text itself replaces earlier versions and is later replaced by the 2013 revision in the same series.

Keywords

lactic acid bacteria, lactic acid microorganisms, food microbiology, dairy microbiology, MPN, plate count, sample preparation, culture media, GOST 10444 series

FAQ

Q: What is this standard?

A: It is a GOST (state/interstate) standard titled "Food products. Methods for determination of lactic acid bacteria" (GOST 10444.11-89) that specifies laboratory methods to detect and enumerate lactic acid bacteria in foodstuffs.

Q: What does it cover?

A: It covers sampling and preparation of samples, composition and preparation of diluents and media, required reagents and apparatus, two principal enumeration techniques (MPN in sterile milk and plate counts on solid media), incubation conditions and calculation/reporting of results.

Q: Who typically uses it?

A: Food testing laboratories, QA/QC teams in dairy and fermented-food producers, regulatory inspectors and researchers performing microbiological analyses related to product safety, quality and shelf life.

Q: Is it current or superseded?

A: The 1989 edition (GOST 10444.11-89) has been superseded by a later revision (GOST 10444.11-2013 / subsequent release of the GOST 10444.11 series). For current regulatory or laboratory practice, users should consult the 2013 revision and any later amendments.

Q: Is it part of a series?

A: Yes — it is part of the GOST 10444.x family of standards addressing microbiological methods for food and animal feeding stuffs; different parts of the series specify methods for different microbial groups and testing procedures.

Q: What are the key keywords?

A: Lactic acid bacteria, food microbiology, dairy products, MPN, plate count, culture media, GOST 10444.11-89.