GOST R 50480-93 PDF

Full title and description

GOST R 50480-93 (ГОСТ Р 50480-93). Title (English): "Food products. Method for detection of Salmonella". Title (Russian): "Продукты пищевые. Метод выявления бактерий рода Salmonella". The standard specifies a culture‑based laboratory method for detection, isolation and preliminary identification of bacteria of the genus Salmonella in defined sample portions of food products.

Abstract

This national (GOST R) microbiological standard describes sampling preparation, required reagents and media, enrichment and selective cultivation procedures, biochemical and serological confirmation steps, and criteria for reporting presence/absence of Salmonella in a tested food sample. It was issued as a technical method for routine food‑safety testing in food control and laboratory practice.

General information

• Status: Withdrawn / not in force (standard marked as not active / cancelled; withdrawn from use effective 01 July 2020).
• Publication date: Approved 1993; introduced (date of entry into force) 01 January 1994.
• Publisher: Gosstandart / Издательство стандартов (issued under the authority of the national standards body of the Russian Federation).
• ICS / categories: ICS 07.100.30 (Microbiology — methods for analysis of food / food microbiology).
• Edition / version: Original GOST R designation: 50480-93 (1993 edition, entered into force 1994).
• Number of pages: Approximately 15 pages (standard text in PDF form typically ~15 pages).

Scope

The standard applies to a wide range of food products and establishes a presence/absence (qualitative) culture‑based procedure for detecting Salmonella in a specified test portion (mass or volume) of food. It covers sample preparation, primary and secondary enrichment, selective plating, colony characterization, biochemical confirmation and serological identification necessary to conclude whether Salmonella is present in the tested sample.

Key topics and requirements

• Sampling and preparation of test portion (defined sample mass/volume, aseptic handling).
• Use of pre‑enrichment and selective enrichment media (e.g., selenite, tetrathionate‑type enrichments) and incubation conditions.
• Plating on differential/selective agars (e.g., bismuth sulfite / Wilson‑Blair, Endo/Levine, or equivalent) and colony morphology criteria for presumptive Salmonella.
• Biochemical confirmation suite (triple‑sugar iron agar, indole, fermentation profiles, motility tests) and interpretive tables for species/subgroup characteristics.
• Serological confirmation (agglutination with specific antisera) and procedures for excluding autoagglutinating strains.
• Reporting rules: formulation of presence/absence statements for Salmonella in the tested mass/volume.

Typical use and users

Used by public health and food control laboratories, quality assurance laboratories in food industry (manufacturers, processors), contract testing laboratories, and research laboratories performing routine microbiological safety testing of foods to detect Salmonella contamination. The method is intended for trained microbiologists familiar with classical culture techniques and biosafety practices for handling enteric pathogens.

Related standards

Closely related and commonly referenced documents include the later intergovernmental/updated designation (ГОСТ 30519-97 / ГОСТ Р 50480-93 references) and national methods for microbiological examination of food (for example, standards for preparation of culture media and general microbiology methods such as ГОСТ 10444.1). Internationally, comparable methods include ISO methods for detection of Salmonella (e.g., ISO 6579 series and subsequent revisions).

Keywords

Salmonella; food microbiology; detection method; enrichment; selective media; biochemical confirmation; serology; presence/absence; GOST R 50480-93; food safety.

FAQ

Q: What is this standard?

A: GOST R 50480-93 is a Russian national (GOST R) standard titled "Food products. Method for detection of Salmonella" that defines a culture‑based laboratory procedure for detecting bacteria of the genus Salmonella in food samples.

Q: What does it cover?

A: It covers sample preparation, required apparatus and reagents, procedures for pre‑enrichment and selective enrichment, selective/differential plating, biochemical and serological confirmation, and rules for reporting presence or absence of Salmonella in a tested portion of food.

Q: Who typically uses it?

A: Food safety laboratories, quality control departments in food production, contract testing labs, and competent authorities performing microbiological safety testing of foods typically use this method. It is aimed at trained laboratory personnel experienced in handling pathogenic bacteria.

Q: Is it current or superseded?

A: The standard is marked as not in force (withdrawn/cancelled); its formal withdrawal date is recorded as 01 July 2020. In practice it has been associated or harmonized with related intergovernmental GOST text (ГОСТ 30519-97 / ГОСТ Р 50480-93) and may have been superseded or replaced in national practice by later standards or by technical regulations and updated microbiological methods. For regulatory use, laboratories should follow the currently active national or international methods adopted by their competent authority.

Q: Is it part of a series?

A: It is part of the body of national GOST/GOST R standards addressing methods for microbiological analysis of food and is cross‑referenced with other microbiology standards (media preparation, general test methods). It has been presented together with or referenced by the intergovernmental designation ГОСТ 30519-97 in the set of food testing standards.

Q: What are the key keywords?

A: Salmonella; food products; detection method; enrichment; selective agar; biochemical confirmation; serology; GOST R; food microbiology.