ISO 10706-2000 PDF

Full title and description

ISO 10706:2000 — Water quality — Determination of long term toxicity of substances to Daphnia magna Straus (Cladocera, Crustacea). This International Standard specifies a laboratory method to assess long‑term (chronic/sublethal) effects of chemicals, effluents and waters on the reproduction and other sublethal endpoints of Daphnia magna.

Abstract

ISO 10706:2000 describes a 21‑day type test for measuring long‑term, sublethal toxicity to Daphnia magna (reproduction and related endpoints). The method is applicable to soluble chemicals, stable suspensions or dispersions, treated/untreated industrial and sewage effluents, and surface or ground waters. NOTE: the standard is adapted from OECD Guideline No. 211 (Daphnia magna reproduction test).

General information

• Status: Published (International Standard; confirmed in ISO systematic reviews).
• Publication date: 30 March 2000 (ISO edition 1:2000).
• Publisher: International Organization for Standardization (ISO).
• ICS / categories: 13.060.70 (Examination of biological properties of water).
• Edition / version: Edition 1 (2000).
• Number of pages: 17 pages.

(General information above based on the ISO bibliographic record and national catalogues.)

Scope

Specifies procedures for conducting a long‑term (typically 21‑day) reproductive toxicity test with Daphnia magna to determine sublethal effects of test substances and waters. The scope covers test organism selection and culturing, test designs (static, semi‑static or flow‑through), control and reference substance use, physical‑chemical monitoring (temperature, dissolved oxygen, pH, hardness), analytical verification of concentrations when required, and reporting of endpoints such as NOEC/LOEC and ECx values. The method is intended to support ecotoxicological hazard assessment and regulatory risk assessment.

Key topics and requirements

• Test organism: Daphnia magna Straus (young females, age criteria at test start).
• Test duration and primary endpoint: 21‑day exposure; reproductive output (number of offspring) and survival as principal measures.
• Test designs: static, semi‑static (renewal) or flow‑through systems; replication and sample size recommendations for statistical power.
• Quality control: use of controls and a reference toxicant to check organism sensitivity and test validity criteria (temperature, oxygen, pH, water hardness limits).
• Concentration verification: analytical measurement of test concentrations for poorly stable or volatile substances.
• Data analysis and reporting: calculation of NOEC/LOEC and ECx (with statistical methods), full reporting of methods, conditions and results for regulatory acceptance.

Typical use and users

Used by environmental laboratories, ecotoxicology contract research organisations, chemical and pesticide registrants, regulators and environmental monitoring agencies to assess chronic aquatic toxicity and inform hazard classification, environmental risk assessment and effluent/water quality evaluations. Laboratories performing GLP or regulatory studies commonly apply this method or the closely related OECD 211 guideline.

Related standards

Closely related guidance and standards include OECD Guideline No. 211 (Daphnia magna reproduction test), ISO 6341 (acute toxicity — inhibition of mobility of Daphnia magna; more recent editions cover acute testing), and other ISO and regional standards addressing aquatic toxicity test methods, reporting and validity criteria. ISO 10706 is explicitly adapted from OECD 211.

Keywords

Daphnia magna; long‑term toxicity; chronic toxicity; reproduction test; water quality; ecotoxicology; NOEC; LOEC; ECx; OECD 211; ISO/TC 147/SC 5.

FAQ

Q: What is this standard?

A: ISO 10706:2000 is an ISO method for determining long‑term (chronic/sublethal) toxicity of substances, effluents and waters to the freshwater cladoceran Daphnia magna, focusing on reproduction and related endpoints.

Q: What does it cover?

A: It covers test design options (static, semi‑static, flow‑through), organism handling and selection, environmental and test‑condition monitoring, validity criteria, analytical verification when needed, and statistical reporting of endpoints such as NOEC, LOEC and ECx for reproductive effects.

Q: Who typically uses it?

A: Environmental toxicology laboratories, CROs running regulatory studies, chemical/pesticide registrants, government regulators and environmental monitoring groups use the standard for chronic aquatic hazard assessment and regulatory submissions.

Q: Is it current or superseded?

A: As published in 2000 (edition 1) ISO 10706:2000 remains the ISO record for this method and has been handled through ISO systematic reviews (listed as confirmed in ISO metadata). Users should check their national standards body for any national adoptions or more recent regional requirements; for related test methods, OECD Guideline 211 (and its updates) is commonly used in regulatory contexts.

Q: Is it part of a series?

A: It belongs to the body of ISO water quality standards (ISO/TC 147 family) covering biological and ecotoxicological test methods; it complements acute‑toxicity methods (e.g., ISO 6341) and other ISO/EN/ASTM methods used together for comprehensive aquatic hazard assessment.

Q: What are the key keywords?

A: Daphnia magna; chronic toxicity; reproduction; water quality; ecotoxicology; NOEC; LOEC; ECx; OECD 211; ISO 10706.