ISO 22662-2024 PDF

Full title and description

ISO 22662:2024 — Milk and milk products — Determination of lactose content by high-performance liquid chromatography (reference method). This second-edition international standard specifies a reference HPLC method for the determination of lactose in a range of milk and dairy matrixes, providing procedures for sample preparation, chromatographic separation, quantification, calculation and expression of results and estimation of uncertainty.

Abstract

ISO 22662:2024 (IDF 198:2024) defines a reference high-performance liquid chromatography (HPLC) procedure for determining lactose content in raw milk, heat‑treated milks, dried milk, dairy permeate and permeate powders, and raw and pasteurized cream. The document sets out sample handling and preparation, chromatographic conditions and calibration principles, criteria for identification and quantification of lactose, expression of results and quality/uncertainty considerations. The method explicitly excludes fermented milks, milks with added oligosaccharides, and low‑lactose or lactose‑free milks.

General information

• Status: Published
• Publication date: 7 March 2024
• Publisher: ISO (International Organization for Standardization) in co-publication / alignment with IDF (International Dairy Federation)
• ICS / categories: 67.100.01 (Milk and milk products — general)
• Edition / version: Edition 2 (2024)
• Number of pages: 10

Scope

This reference standard specifies an HPLC‑based method for determination of lactose content in: raw milk, heat‑treated milks, dried milk, dairy permeate and dairy permeate powder, and raw and pasteurized cream. It is intended as a reference procedure for laboratories performing definitive lactose determinations and for method validation/comparison. The method does not apply to fermented milks, milks with added oligosaccharides, or products marketed as low‑lactose or lactose‑free.

Key topics and requirements

• Definition of the target analyte (lactose) and applicable sample types and exclusions.
• Detailed sample preparation and pre‑treatment procedures designed to remove interferences and ensure representative aliquots.
• Chromatographic separation principles and parameters (HPLC approach) for reliable identification of lactose peaks and separation from other carbohydrates.
• Calibration and use of appropriate reference materials/standards for quantification, including blank and calibration checks.
• Requirements for peak identification, integration and criteria for acceptance of chromatograms.
• Procedures for result calculation, reporting (units, significant figures) and expression on dry matter or as-is basis where relevant.
• Performance characteristics and quality control: repeatability, reproducibility, limits of application and estimates of measurement uncertainty.
• Guidance for verification, interlaboratory comparison and use as a reference method for method validation.

Typical use and users

Laboratories performing official or reference analyses of milk and dairy products, national and industrial quality control laboratories, regulatory and conformity assessment bodies, method validation teams, and research groups in dairy chemistry. The standard is used where accurate, traceable lactose results are required for product labelling, regulatory compliance, nutritional analysis and method development/validation.

Related standards

This edition replaces ISO 22662:2007 (previous edition) and is published in alignment with IDF 198:2024. Laboratories commonly use ISO 22662 alongside other dairy analytical reference standards (for example standards for compositional analysis and instrumental methods such as mid‑infrared-based methods) when establishing comprehensive testing schemes and accreditation scopes.

Keywords

lactose; milk; dairy products; HPLC; high-performance liquid chromatography; reference method; IDF 198; lactose determination; analytical method; measurement uncertainty; sample preparation; quality control.

FAQ

Q: What is this standard?

A: ISO 22662:2024 is the second‑edition international reference standard specifying an HPLC method for the determination of lactose content in defined milk and dairy matrices.

Q: What does it cover?

A: It covers sample preparation, chromatographic analysis, calibration, identification and quantification of lactose, calculation and expression of results, and estimation of measurement uncertainty for raw milk, heat‑treated milks, dried milk, dairy permeate and permeate powders, and raw and pasteurized cream. It excludes fermented milks, milks with added oligosaccharides, and low‑lactose or lactose‑free milks.

Q: Who typically uses it?

A: Reference and accredited testing laboratories, dairy industry QC/technical teams, regulatory agencies, and researchers who need a validated, traceable method for lactose analysis or who are validating alternative methods against a reference procedure.

Q: Is it current or superseded?

A: Current — ISO 22662:2024 is the active edition (second edition) and supersedes ISO 22662:2007.

Q: Is it part of a series?

A: It is published as ISO 22662 and jointly referenced as IDF 198 (the international dairy federation identifier). It is typically used alongside other ISO/IDF standards for milk and milk product analysis and for laboratories’ accreditation scopes.

Q: What are the key keywords?

A: Lactose, HPLC, milk, dairy products, reference method, IDF 198, analytical chemistry, calibration, measurement uncertainty.