ISO 24465-2023 PDF

Full title and description

ISO 24465:2023 — Surface chemical analysis — Determination of the minimum detectability of surface plasmon resonance device. The standard specifies a standardized method to determine the lowest detectable signal (minimum detectability) of surface plasmon resonance (SPR) devices and is intended for devices using white‑light or laser illumination with angle‑scanning capability.

Abstract

This document describes a method for determining the minimum detectability (lower limit of detection) of surface plasmon resonance devices. It is applicable to SPR instruments of the white‑light illumination type and laser illumination type when they have angle‑scanning capability; the procedure is designed to minimize unwanted surface–analyte interactions and is suitable for use by non‑expert operators.

General information

• Status: Published.
• Publication date: January 2023 (published 10 January 2023).
• Publisher: International Organization for Standardization (ISO).
• ICS / categories: 71.040.40 (surface chemical analysis).
• Edition / version: Edition 1 (2023).
• Number of pages: 9 pages (official ISO publication).

Scope

ISO 24465:2023 establishes a laboratory procedure and definitions needed to measure the minimum detectability (lower limit of detection) of surface plasmon resonance devices. The scope explicitly covers SPR instruments using either white‑light illumination or laser illumination provided they support angle scanning; the method uses a single‑solute approach to reduce complex surface chemistry effects and to make the test accessible to non‑specialist operators.

Key topics and requirements

• Definition and standardized metric(s) for minimum detectability / lower limit of detection for SPR instruments.
• Test procedure using a single chemical solute to avoid unwanted interactions with the metal surface.
• Applicability to white‑light and laser illumination SPR devices with angle‑scanning capability.
• Data acquisition and analysis steps sufficient to determine the instrument’s lowest detectable signal (practical LOD).
• Guidance intended to be executable by non‑expert users while providing reproducible performance assessment for manufacturers, testers and laboratories.

Typical use and users

Typical users include SPR instrument manufacturers (for device characterization and quality control), analytical and surface‑science laboratories, biosensor developers, academic researchers working on surface binding and kinetics, and QA/QC staff who need a standard method to compare instrument sensitivity or verify performance claims. The standard is positioned within ISO/TC 201’s suite of surface chemical analysis documents.

Related standards

ISO 24465:2023 is part of the ISO/TC 201 (surface chemical analysis) domain. Other ISO/TC 201 standards cover related surface‑analysis techniques and procedures (examples include ISO documents on SIMS, XPS and general surface analysis procedures such as ISO 18114 and earlier deliverables referenced by TC 201); users working with SPR commonly consult those TC 201 references for broader surface‑analysis context.

Keywords

surface plasmon resonance; SPR; minimum detectability; limit of detection; lower limit of detection; surface chemical analysis; analytical performance; angle scanning; white‑light SPR; laser‑illumination SPR; ISO/TC 201.

FAQ

Q: What is this standard?

A: ISO 24465:2023 is an international standard that defines a method for determining the minimum detectability (lower limit of detection) of surface plasmon resonance (SPR) devices.

Q: What does it cover?

A: It covers a standardized test procedure and definitions to assess the lowest detectable signal of SPR instruments, applicable to white‑light and laser illumination instruments with angle‑scanning capability, and provides a single‑solute protocol to limit surface chemistry complications.

Q: Who typically uses it?

A: Instrument manufacturers, analytical and surface‑analysis laboratories, biosensor developers, academic researchers and QA/QC personnel who need reproducible, comparable measures of SPR sensitivity.

Q: Is it current or superseded?

A: It is a current, published International Standard (first edition, published January 2023). There is no indication in the ISO catalogue that it has been superseded as of its publication record.

Q: Is it part of a series?

A: It is part of the body of standards developed under ISO/TC 201 (surface chemical analysis); while not a numbered sub‑part series, it complements other TC 201 standards covering surface analysis techniques and procedures.

Q: What are the key keywords?

A: Surface plasmon resonance (SPR), minimum detectability, limit of detection, surface chemical analysis, angle scanning, white‑light, laser illumination, ISO/TC 201.