ISO 10095-1992 PDF

Full title and description

ISO 10095:1992 — Coffee — Determination of caffeine content — Method using high-performance liquid chromatography. This International Standard describes an HPLC-based analytical procedure for extracting and quantifying caffeine in green and roasted coffee, decaffeinated products and coffee extracts.

Abstract

This standard specifies the principle, reagents, apparatus, sampling and preparation of the test sample, the test procedure, expression of results, precision and the test report for determining caffeine content in coffee by high‑performance liquid chromatography (HPLC). The method includes sample extraction, purification on a small silica/phenyl column and UV detection of caffeine.

General information

• Status: Withdrawn / not current.
• Publication date: January 1992 (Edition 1).
• Publisher: International Organization for Standardization (ISO).
• ICS / categories: 67.140.20 (Coffee and coffee substitutes).
• Edition / version: 1st edition, 1992.
• Number of pages: 6 pages (as listed in the ISO bibliographic record).

Scope

Applies to the determination of caffeine in green coffee, roasted coffee, decaffeinated coffees and powdered or liquid coffee extracts. The procedure covers extraction of caffeine from the test portion (including an extraction step using hot water), cleanup on a micro-column (silica with a phenyl group), and quantification by HPLC with UV detection; it specifies reagents, apparatus, sampling/preparation, calculation and reporting requirements.

Key topics and requirements

• HPLC-based quantification of caffeine with ultraviolet detection.
• Sample extraction and preparation (hot-water extraction step referenced in the method).
• Purification/clean-up using a small silica/phenyl micro-column prior to chromatographic analysis.
• Specification of reagents, apparatus and chromatographic conditions, plus precision and expression of results.
• Reporting format and test-report content required for laboratory records and results exchange.

Typical use and users

Used by analytical laboratories, coffee processors, quality control departments, research institutions and regulatory agencies concerned with accurate measurement of caffeine in coffee products and extracts. Although useful as an HPLC procedural reference, users should note the standard is withdrawn and verify whether national or more recent validated methods are required for compliance or regulatory purposes.

Related standards

ISO 4052:1983 (Reference method — determination of caffeine content in coffee) is a closely related ISO reference procedure for caffeine determination; both documents have appeared in ISO's lifecycle as withdrawn items in later reviews. Users commonly also consult national methods and AOAC/OA analytical methods for caffeine in foods and beverages when selecting a validated method.

Keywords

caffeine; coffee; high-performance liquid chromatography; HPLC; determination; analytical method; coffee extracts; decaffeinated coffee; sample preparation; ISO 10095:1992.

FAQ

Q: What is this standard?

A: ISO 10095:1992 is an ISO International Standard that defines a method using high‑performance liquid chromatography (HPLC) to determine caffeine content in coffee and coffee extracts.

Q: What does it cover?

A: It covers the analytical principle, required reagents and apparatus, sampling and sample preparation, purification of extracts (micro-column), chromatographic determination by HPLC with UV detection, expression of results, precision and the required test report.

Q: Who typically uses it?

A: Analytical chemistry laboratories in the coffee industry, quality control teams, research labs and regulators interested in measuring caffeine in green and roasted coffee, decaffeinated products and coffee extracts.

Q: Is it current or superseded?

A: ISO 10095:1992 is listed as withdrawn and is not a current active ISO standard; users should not assume it represents the latest validated method and should check current national or international guidance before using it for compliance testing.

Q: Is it part of a series?

A: It is part of the body of ISO standards on coffee analysis produced by ISO/TC 34 (Agricultural food products), and is related to other ISO methods for caffeine determination (for example ISO 4052). Laboratories often consider ISO 10095 alongside reference and national methods when developing or validating in‑house procedures.

Q: What are the key keywords?

A: caffeine, coffee, HPLC, high-performance liquid chromatography, determination, analytical method, coffee extracts, decaffeinated, ISO 10095.