ISO 10315-2021 PDF

Full title and description

ISO 10315:2021 — Cigarettes — Determination of nicotine in total particulate matter from the mainstream smoke — Gas‑chromatographic method. This international standard specifies a GC-based analytical procedure for measuring nicotine in the total particulate matter (TPM) collected from mainstream cigarette smoke; smoking and TPM collection are carried out in accordance with ISO 4387.

Abstract

This document specifies a method for the gas‑chromatographic determination of nicotine in total particulate matter from mainstream cigarette smoke. It describes sample collection (TPM), sample preparation/extraction, chromatographic determination using an internal standard, and reporting of results. The standard notes related methods for intense smoking regimes (ISO 20778 and ISO 22253).

General information

• Status: Published.
• Publication date: 2021-06-01 (June 1, 2021).
• Publisher: International Organization for Standardization (ISO).
• ICS / categories: 65.160 (Tobacco, tobacco products and related equipment).
• Edition / version: Edition 4 (2021).
• Number of pages: 9 pages.

Scope

ISO 10315:2021 applies to the determination of nicotine in the total particulate matter of mainstream cigarette smoke using a gas‑chromatographic method. It covers the collection of mainstream smoke condensate (TPM) in accordance with ISO 4387 and the analytical steps required to extract, analyse and report nicotine content. The standard is intended for routine laboratory determinations of nicotine delivered per cigarette under the referenced smoking regime.

Key topics and requirements

• Sampling and TPM collection according to ISO 4387 (smoking machine and condensation procedures).
• Sample preparation and extraction of nicotine from the particulate matter (solvent extraction prior to GC).
• Gas‑chromatographic separation and detection parameters, including use of an internal standard for quantification.
• Calibration, quality control and calculation of nicotine content per cigarette (reporting conventions).
• Scope limitations and cross‑references to alternative/intense smoking regime methods (ISO 20778, ISO 22253).

Typical use and users

Laboratories performing tobacco product testing, regulatory and conformity assessment bodies, quality departments within tobacco manufacturers, independent testing laboratories, and research organizations studying smoke chemistry or product emissions use this standard to obtain reproducible, comparable nicotine measurements from mainstream smoke TPM.

Related standards

Key related standards include ISO 4387 (smoking and collection of mainstream smoke/TPM), ISO 20778 and ISO 22253 (methods for nicotine determination under intense smoking regimes). Other tobacco‑testing standards and guidance produced by national bodies (e.g., corresponding national adoptions) may also be used alongside ISO 10315:2021.

Keywords

nicotine, cigarettes, mainstream smoke, total particulate matter (TPM), gas chromatography, smoke condensate, ISO 4387, tobacco testing, analytical method, quality control.

FAQ

Q: What is this standard?

A: ISO 10315:2021 is an international standard that specifies a gas‑chromatographic method for determining nicotine in the total particulate matter collected from mainstream cigarette smoke.

Q: What does it cover?

A: It covers TPM collection (as per ISO 4387), sample preparation/extraction, GC analysis with an internal standard, calibration and reporting of nicotine results (expressed per cigarette). It also points to alternative methods for intense smoking regimes (ISO 20778, ISO 22253).

Q: Who typically uses it?

A: Tobacco testing laboratories, regulatory agencies, manufacturers' quality/control teams, and research institutions performing chemical analysis of cigarette smoke.

Q: Is it current or superseded?

A: ISO 10315:2021 (Edition 4) is the current published edition (published 1 June 2021). It replaced earlier editions (for example 2013 and prior) and, according to ISO tracking, is under systematic review on the normal ISO 5‑year cycle. Check the ISO catalogue or national mirror bodies for any amendments or confirmations.

Q: Is it part of a series?

A: It is part of the body of ISO standards for tobacco and tobacco‑product testing maintained by ISO/TC 126. It links to related standards that address TPM collection (ISO 4387) and nicotine determination under different smoking regimes (ISO 20778, ISO 22253).

Q: What are the key keywords?

A: Nicotine; cigarettes; mainstream smoke; total particulate matter; gas chromatography; smoke condensate; tobacco testing; ISO 4387.