ISO 6464-1983 PDF

Full title and description

Animal and vegetable fats and oils — Determination of gallates content — Molecular absorption spectrometric method (ISO 6464:1983). Specifies a UV (molecular absorption) spectrometric procedure for the separate determination of propyl, octyl and dodecyl gallates used as antioxidants in animal and vegetable fats and oils.

Abstract

ISO 6464:1983 defines a selective-extraction, ultraviolet spectrometric method to quantify propyl gallate, octyl gallate and dodecyl gallate in fats and oils. The method uses appropriate solvents to extract each gallate from a test portion and measures absorbance by molecular (UV) absorption spectrometry. The standard notes that other antioxidants identifiable by ISO 5558 may interfere and give elevated results.

General information

• Status: Withdrawn (withdrawal effective 29 November 2006).
• Publication date: 1 November 1983 (Edition 1, 1983).
• Publisher: International Organization for Standardization (ISO).
• ICS / categories: 67.200.10 — Animal and vegetable fats and oils.
• Edition / version: Edition 1 (1983).
• Number of pages: 3.

Scope

Applies to the determination of specific gallate antioxidants (propyl, octyl and dodecyl gallates) in animal and vegetable fats and oils by molecular (UV) absorption spectrometry following selective solvent extraction. The scope excludes cases where other antioxidants (detectable/identifiable by ISO 5558) interfere with the measurement; such interferences can lead to erroneous high results.

Key topics and requirements

• Principle: selective extraction of individual gallates from a test portion followed by quantification by UV molecular absorption spectrometry.
• Analytes covered: propyl gallate, octyl gallate and dodecyl gallate (separate determination in that order).
• Interference considerations: other antioxidants identifiable by ISO 5558 may interfere and must be considered.
• Reagents and solvents: requires analytical-grade reagents and appropriate extraction solvents (e.g., non-polar solvents for fats/oils extraction).
• Instrumental requirement: UV/molecular absorption spectrometer suitable for the absorption maxima of the targeted gallates and capable of accurate quantitative measurements.
• Reporting: results expressed according to the method’s instructions and test-report data requirements (sample preparation, extraction details, instrument conditions, limits of detection/quantification where applicable).

Typical use and users

Used historically by analytical laboratories in the edible oils and fats industry, food control and regulatory laboratories, research institutes and quality-control departments of manufacturers of edible oils, margarine, shortening and related products that required measurement of gallate-type antioxidants.

Related standards

Closely related to ISO 5558 (methods for detection/identification of antioxidants) and to other ISO methods for fats and oils analysis (for example standards covering acid value, peroxide value and chromatographic preparation of fatty acid methyl esters). National/adopted equivalents and related documents include NFT 60‑238:1984 (France) and corresponding national listings/identical adoptions in various countries.

Keywords

gallates; propyl gallate; octyl gallate; dodecyl gallate; fats and oils; antioxidants; UV spectrometry; molecular absorption; extraction; analytical method.

FAQ

Q: What is this standard?

A: ISO 6464:1983 is an International Standard that specified a molecular (UV) absorption spectrometric method for the separate determination of propyl, octyl and dodecyl gallates in animal and vegetable fats and oils.

Q: What does it cover?

A: It covers selective solvent extraction of each gallate from a test portion and their quantification by UV spectrometry, with notes on possible interference from other antioxidants detectable by ISO 5558.

Q: Who typically uses it?

A: Analytical and quality-control laboratories in the edible fats and oils sector, food-testing laboratories, research institutions and regulatory bodies involved in compositional testing of fats and oils.

Q: Is it current or superseded?

A: ISO 6464:1983 has been withdrawn (withdrawal effective 29 November 2006). It is not a current active ISO method; users should consult the ISO catalogue or current national standards for updated or replacement methods for antioxidant determination in fats and oils.

Q: Is it part of a series?

A: It is one of a set of analytical methods for animal and vegetable fats and oils developed under ISO/TC 34/SC 11. It is related to other ISO methods on antioxidants and general fat-and-oil analysis (for example ISO 5558 and other ISO methods for oil testing).

Q: What are the key keywords?

A: Gallates, propyl gallate, octyl gallate, dodecyl gallate, antioxidant analysis, UV spectrometry, fats and oils, extraction, analytical method.